ProC-RNA (Protein Co-localizer on RNA)

 

 

Once RNAs have been transcribed, RNA-binding proteins (RBPs) play an important role in regulating their alternative splicing, stability, transport and translation. These processes are often regulated by groups of proteins that bind in the proximity of each other on RNA.

 

CLIP-seq has been used to map RNA-binding sites for a large number of RBPs and these datasets can be leveraged to understand how RBPs may cooperate with each other to regulate gene expression.

 

ProC-RNA uses statistical methods to screen a large collection of CLIP-seq experiments to identify the set of RBPs that most significantly co-localize with your RBP of interest (ROI) on RNA.

The output of this analysis will include a list of RBPs that are ranked based on their significance of co-localization with your ROI along with associated statistics, as well as the RNA-binding sites where these proteins co-localize.

 

This analysis can help you identify other proteins which you ROI may cooperate to regulate various stages of gene expression and it can help you design experiments to test their cooperation.

 

If you have an RNA-binding protein of interest that you would like to analyze with ProC-RNA, you can provide us with one of the following:

 

  • The locations where you RBP of interest binds RNA.

  • The raw CLIP-seq data for you RBP of interest, or other sequencing based methods that identify association of proteins with RNA.

  • Contact us to inquire if we have already found high quality datasets that provide the locations where your RBP of interest binds RNA.

 

 

Please contact us to inquire about how ProC-RNA works, how it can be tailored to your needs, as well as quotes.